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The mitochondrial genome of the pathogenic yeast Candida subhashii: GC-rich linear DNA with a protein covalently attached to the 5′ termini

机译：致病性酵母Subhashii的线粒体基因组：富含GC的线性DNA，其蛋白质与5'末端共价连接

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As a part of our initiative aimed at a large-scale comparative analysis of fungal mitochondrial genomes, we determined the complete DNA sequence of the mitochondrial genome of the yeast Candida subhashii and found that it exhibits a number of peculiar features. First, the mitochondrial genome is represented by linear dsDNA molecules of uniform length (29 795 bp), with an unusually high content of guanine and cytosine residues (52.7 %). Second, the coding sequences lack introns; thus, the genome has a relatively compact organization. Third, the termini of the linear molecules consist of long inverted repeats and seem to contain a protein covalently bound to terminal nucleotides at the 5′ ends. This architecture resembles the telomeres in a number of linear viral and plasmid DNA genomes classified as invertrons, in which the terminal proteins serve as specific primers for the initiation of DNA synthesis. Finally, although the mitochondrial genome of C. subhashii contains essentially the same set of genes as other closely related pathogenic Candida species, we identified additional ORFs encoding two homologues of the family B protein-priming DNA polymerases and an unknown protein. The terminal structures and the genes for DNA polymerases are reminiscent of linear mitochondrial plasmids, indicating that this genome architecture might have emerged from fortuitous recombination between an ancestral, presumably circular, mitochondrial genome and an invertron-like element.
机译：作为旨在对真菌线粒体基因组进行大规模比较分析的计划的一部分，我们确定了酵母哈苏假丝酵母线粒体基因组的完整DNA序列，并发现其具有许多独特的功能。首先，线粒体基因组由具有相同长度（29DNA795 bp）的线性dsDNA分子代表，鸟嘌呤和胞嘧啶残基的含量异常高（52.7％）。其次，编码序列缺乏内含子。因此，基因组具有相对紧凑的组织。第三，线性分子的末端由长的反向重复组成，并且似乎包含与5'末端的末端核苷酸共价结合的蛋白质。这种结构类似于许多归类为内转子的线性病毒和质粒DNA基因组中的端粒，其中末端蛋白用作DNA合成起始的特异性引物。最后，尽管C.subhashii的线粒体基因组与其他密切相关的致病性念珠菌属物种基本包含相同的基因集，但我们鉴定出了编码B族蛋白引发DNA聚合酶的两个同系物和一个未知蛋白的其他ORF。 DNA聚合酶的末端结构和基因使人联想到线性线粒体质粒，这表明这种基因组结构可能是由祖先的，可能是环状的线粒体基因组和一个类似反向子的元素之间的偶然重组产生的。

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